Yeast DNA replication.

DNA Replication in Yeast

The details of chromosome replication are better understood in the budding yeast, Saccharomyces cerevisiae, than in any other eukaryotic organism. cis-Acting replicator sequences required for chromosomal replication origin function were identified on the basis of their ability to promote the extrachromosomal maintenance of plasmids. These autonomously replicating sequence (ARS) elements have be...

Dynamics of DNA replication in yeast.

We present a mathematical model for the spatial dynamics of DNA replication. Using this model we determine the probability distribution for the time at which each chromosomal position is replicated. From this we show, contrary to previous reports, that mean replication time curves cannot be used to directly determine origin parameters. We demonstrate that the stochastic nature of replication dy...

Role of yeast DNA polymerase epsilon during DNA replication

DNA Replication: Mammalian Treslin–TopBP1 Interaction Mirrors Yeast Sld3–Dpb11

There are many parallels between DNA replication in yeast and humans. Now, two recent studies extend this relationship by dissecting key conserved interactions necessary for initiation of the replisome.

Organization of DNA replication origins in the fission yeast genome.

Eukaryotic DNA replication initiates at multiple points along the chromosomes known as replication origins (ORIs). We have developed a strategy to identify ORIs directly from replication intermediates in the fission yeast Schizosaccharomyces pombe. Mapping of a selection of the novel ORIs onto the genome reveals their preferential localization at intergenic regions upstream from genes. These re...